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Image Search Results
Journal: JAIDS Journal of Acquired Immune Deficiency Syndromes
Article Title: Increased Sensitivity of HIV-1 p24 ELISA Using a Photochemical Signal Amplification System
doi: 10.1097/qai.0000000000000726
Figure Lengend Snippet: FIGURE 2. Calibration curves for determination of HIV-1 p24 antigen using non-ICD ELISA + PSAM at dif- ferent times of illumination (A and B) as compared with conventional non- ICD ELISA (B, closed squares). The calibration curve obtained after 12 mi- nutes of illumination (A, closed circles) provides the highest sensitivity of the assay, with a lower limit of detection of 0.08 pg/mL. Curves represented by open and closed triangles (B) corre- spond to signal-amplified ELISA at 0 and 4 minutes of illumination and can be used to calculate high HIV-1 p24 antigen concentrations.
Article Snippet: We also excluded the tyramide signal amplification (TSA) step15 that requires the use of
Techniques: Enzyme-linked Immunosorbent Assay
Journal: JAIDS Journal of Acquired Immune Deficiency Syndromes
Article Title: Increased Sensitivity of HIV-1 p24 ELISA Using a Photochemical Signal Amplification System
doi: 10.1097/qai.0000000000000726
Figure Lengend Snippet: FIGURE 3. Calibration curves for determination of HIV-1 p24 antigen using heat-mediated ICD ELISA + PSAM at different times of illumination (A and B) as compared with conventional heat- mediated ICD ELISA (B, closed squares). The calibration curve obtained after 9 minutes of illumination (A, closed circles) provides the highest sensitivity, with a lower limit of detection of approximately 0.1 pg/mL. Curves rep- resented by open and closed triangles (B) correspond to signal-amplified ELISA at 0 and 4 minutes of illumination and can be used to calculate high HIV-1 p24 antigen concentrations.
Article Snippet: We also excluded the tyramide signal amplification (TSA) step15 that requires the use of
Techniques: Enzyme-linked Immunosorbent Assay
Journal:
Article Title: The immunosuppressive activity of Ascaris suum is due to high molecular weight components
doi: 10.1046/j.1365-2249.1998.00723.x
Figure Lengend Snippet: Antibody isotypes produced against ovalbumin (OvA) by OvA, OvA + Ascaris suum body extract (Asc), OvA + PI or OvA + PIII-immunized or normal DBA/2 mice. Isotype levels in immune plasma obtained after 9 days of immunization or normal plasma (control) were titrated by ELISA using monospecific antisera. The results are expressed as the mean of five to six samples/group (+ s.e.m.), in 1:512 (IgG1) or 1:8 (IgG2a, IgM and IgE) dilution. *P < 0.05 compared with OvA-immunized group. OD, Optical density.
Article Snippet: The sensitivity of IL-4 assays was increased by using the
Techniques: Produced, Clinical Proteomics, Control, Enzyme-linked Immunosorbent Assay
Journal:
Article Title: The immunosuppressive activity of Ascaris suum is due to high molecular weight components
doi: 10.1046/j.1365-2249.1998.00723.x
Figure Lengend Snippet: Antibody isotypes produced against Ascaris suum body extract (Asc) by ovalbumin (OvA) + PI or OvA + PIII-immunized or normal DBA/2 mice. Isotype levels in immune plasma obtained after 9 days of immunization or normal plasma (control) were titrated by ELISA using monospecific antisera. The results are expressed as the mean of five to six samples/group (+ s.e.m.), in 1:128 (IgG1), 1:256 (IgG2a), or 1:16 (IgE) dilution. Upper right: IgE antibody titres obtained by passive cutaneous anaphylactic (PCA) reaction in pools of plasma from OvA + PI- or OvA + PIII-immunized mice. *P < 0.05 compared with PI-immunized group. OD, Optical density.
Article Snippet: The sensitivity of IL-4 assays was increased by using the
Techniques: Produced, Clinical Proteomics, Control, Enzyme-linked Immunosorbent Assay